Acetylcholinesterase/ACHE

English Name: ACHE; Acetylcholinesterase; Acetylcholine acetylhydrolase; Cholinesterase, Acetyl
CAS Number: 9000-81-1

Grade: BR
Activity: ≥200 units/g protein

Definition of Enzyme Activity Unit: One unit of acetylcholinesterase activity is defined as the amount of enzyme that hydrolyzes 1 μmol of acetylthiocholine iodide (ATCI) per minute under the conditions of 37°C and 0.1 mol/L phosphate buffer at pH=8.0.

Appearance (The following information is for reference only): Off-white or brown powder. Sourced from the heads of flies highly sensitive to pesticides. Under certain conditions, organophosphorus and carbamate pesticides inhibit the normal function of cholinesterase, with the inhibition rate proportional to pesticide concentration. Normally, the enzyme catalyzes the hydrolysis of the neural metabolite acetylcholine, and the hydrolysis products react with a chromogenic agent to produce a yellow substance. The change in absorbance over time is measured using a spectrophotometer to calculate the inhibition rate. The presence of organophosphorus and carbamate pesticides in products can be detected through the inhibition rate.
Use: This product is for research use only and not for other purposes. (The following uses are for reference only) Used in various instruments and spectrophotometers for detecting organophosphorus and carbamate pesticide residues in vegetables and fruits by the enzyme inhibition rate method.
Storage: 2～8°C